In Saccharomyces cerevisiae the FOB1 gene affects replication fork blocking activity at the replication fork block (RFB) sequences and promotes recombination events within the rDNA cluster. Using in vivo footprinting assays we mapped two in vivo Fob1p-binding sites, RFB1 and RFB3, located in the rDNA enhancer region and coincident with those previously reported to be in vitro binding sites. We previously provided evidences that DNA topoisomerase I is able to cleave two sites within this region. The results reported in this paper, indicate that the DNA topoisomerase I cleavage specific activity at the enhancer region is affected by the presence of Fob1p and independent of replication and transcription activities. We thus hypothesize that the binding to DNA of Fob1p itself may be the cause of the DNA topoisomerase I activity in the rDNA enhancer.

FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the S.cerevisiae ribosomal DNA locus / DI FELICE, Francesca; Francesco, Cioci; Camilloni, Giorgio. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 33:19(2005), pp. 6327-6337. [10.1093/nar/gki950]

FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the S.cerevisiae ribosomal DNA locus.

DI FELICE, Francesca;CAMILLONI, Giorgio
2005

Abstract

In Saccharomyces cerevisiae the FOB1 gene affects replication fork blocking activity at the replication fork block (RFB) sequences and promotes recombination events within the rDNA cluster. Using in vivo footprinting assays we mapped two in vivo Fob1p-binding sites, RFB1 and RFB3, located in the rDNA enhancer region and coincident with those previously reported to be in vitro binding sites. We previously provided evidences that DNA topoisomerase I is able to cleave two sites within this region. The results reported in this paper, indicate that the DNA topoisomerase I cleavage specific activity at the enhancer region is affected by the presence of Fob1p and independent of replication and transcription activities. We thus hypothesize that the binding to DNA of Fob1p itself may be the cause of the DNA topoisomerase I activity in the rDNA enhancer.
2005
01 Pubblicazione su rivista::01a Articolo in rivista
FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the S.cerevisiae ribosomal DNA locus / DI FELICE, Francesca; Francesco, Cioci; Camilloni, Giorgio. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 33:19(2005), pp. 6327-6337. [10.1093/nar/gki950]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/17769
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 18
social impact