Nop56p was initially identified in yeast as the third common component of the ribonucleoprotein particles (snoRNPs) assembled on box C/D small nucleolar RNAs (snoRNAs). Thereafter, the characterization of Nop56p homologs in Archaea and in several eukaryotes pointed to the highly conserved structure of this factor. Studies in yeast indicate that Nop56 is not required for the stability of box C/D snoRNAs. Through the isolation of a Xenopus laevis Nop56 cDNA clone, we have been able to characterize the X. laevis Nop56 protein (XNop56p). We showed that it is a common component of X. laevis box C/D snoRNPs and that it displays the same electrophoretic mobility of p62 protein that we previously characterized as a box C/D snoRNP component, not essential for snoRNA stability in X. laevis. Mapping the 5' end of X. laevis Nop56 transcript indicates that it starts with a pyrimidine tract and the analysis of genomic clones revealed a snoRNA encoded in one of NOP56 introns. Although these two characteristics could suggest that XNOP56 is a TOP gene, it is not translationally controlled in a growth-dependent manner

Characterization of the sequences encoding for X.laevis box C/D snoRNP Nop56 protein / Renzi, Fabiana; Filippini, D; Loreni, F; Bozzoni, Irene; Caffarelli, E.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1575:(2002), pp. 26-30. [10.1016/S0167-4781(02)00233-6]

Characterization of the sequences encoding for X.laevis box C/D snoRNP Nop56 protein.

RENZI, FABIANA;BOZZONI, Irene;
2002

Abstract

Nop56p was initially identified in yeast as the third common component of the ribonucleoprotein particles (snoRNPs) assembled on box C/D small nucleolar RNAs (snoRNAs). Thereafter, the characterization of Nop56p homologs in Archaea and in several eukaryotes pointed to the highly conserved structure of this factor. Studies in yeast indicate that Nop56 is not required for the stability of box C/D snoRNAs. Through the isolation of a Xenopus laevis Nop56 cDNA clone, we have been able to characterize the X. laevis Nop56 protein (XNop56p). We showed that it is a common component of X. laevis box C/D snoRNPs and that it displays the same electrophoretic mobility of p62 protein that we previously characterized as a box C/D snoRNP component, not essential for snoRNA stability in X. laevis. Mapping the 5' end of X. laevis Nop56 transcript indicates that it starts with a pyrimidine tract and the analysis of genomic clones revealed a snoRNA encoded in one of NOP56 introns. Although these two characteristics could suggest that XNOP56 is a TOP gene, it is not translationally controlled in a growth-dependent manner
2002
cDNA library; Nop56 protein; Small nucleolar RNA; Small nucleolar ribonucleoprotein particle; TOP gene; Translational regulation; Xenopus laevis
01 Pubblicazione su rivista::01a Articolo in rivista
Characterization of the sequences encoding for X.laevis box C/D snoRNP Nop56 protein / Renzi, Fabiana; Filippini, D; Loreni, F; Bozzoni, Irene; Caffarelli, E.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1575:(2002), pp. 26-30. [10.1016/S0167-4781(02)00233-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/15337
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