The activation of human platelets by polymorphonuclear leukocytes (PMN) was investigated in human whole blood challenged with "priming" concentrations of arachidonic acid or collagen in the presence or absence of N-formyl-Met-Leu-Phe (FMLP), a selective activator of PMN. With the use of arachidonic acid or collagen alone at priming concentrations or FMLP alone, no platelet response was observed. In contrast, FMLP in combination with arachidonic acid or collagen caused irreversible platelet aggregation with thromboxane A2 production. Platelet response to FMLP-activated PMN was enhanced by superoxide dismutase and blocked by catalase or the NADPH oxidase inhibitor diphenyliodonium, suggesting a role for the O2-.-H2O2 system in this cellular interaction. This was corroborated by experiments with exogenously added H2O2, which mimicked FMLP effects in the activation of primed platelets in whole blood. The present investigation indicates that platelets primed with minute amounts of arachidonic acid or collagen can be activated, in human whole blood, by oxygen-reactive species released by PMN.

Polymorphonuclear leukocyte-derived o2-reactive species activate platelets in human whole blood / Pratico, D; Iuliano, Luigi; Alessandri, Cesare; Camastra, C; Violi, Francesco. - In: AMERICAN JOURNAL OF PHYSIOLOGY. - ISSN 0002-9513. - STAMPA. - 264:5(1993), pp. 1582-1587.

Polymorphonuclear leukocyte-derived o2-reactive species activate platelets in human whole blood.

IULIANO, Luigi;ALESSANDRI, Cesare;VIOLI, Francesco
1993

Abstract

The activation of human platelets by polymorphonuclear leukocytes (PMN) was investigated in human whole blood challenged with "priming" concentrations of arachidonic acid or collagen in the presence or absence of N-formyl-Met-Leu-Phe (FMLP), a selective activator of PMN. With the use of arachidonic acid or collagen alone at priming concentrations or FMLP alone, no platelet response was observed. In contrast, FMLP in combination with arachidonic acid or collagen caused irreversible platelet aggregation with thromboxane A2 production. Platelet response to FMLP-activated PMN was enhanced by superoxide dismutase and blocked by catalase or the NADPH oxidase inhibitor diphenyliodonium, suggesting a role for the O2-.-H2O2 system in this cellular interaction. This was corroborated by experiments with exogenously added H2O2, which mimicked FMLP effects in the activation of primed platelets in whole blood. The present investigation indicates that platelets primed with minute amounts of arachidonic acid or collagen can be activated, in human whole blood, by oxygen-reactive species released by PMN.
1993
platelet aggregation, thrombosis
01 Pubblicazione su rivista::01a Articolo in rivista
Polymorphonuclear leukocyte-derived o2-reactive species activate platelets in human whole blood / Pratico, D; Iuliano, Luigi; Alessandri, Cesare; Camastra, C; Violi, Francesco. - In: AMERICAN JOURNAL OF PHYSIOLOGY. - ISSN 0002-9513. - STAMPA. - 264:5(1993), pp. 1582-1587.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/15291
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