Recent findings indicate a significant association between sedentary (SED)-time and type 2 diabetes mellitus(T2DM). The aim of this study was to investigate whether different levels of SED-time could impact on biochemical and physiological processes occurring in sedentary and physically inactive T2DM patients. In particular, patients from the "Italian Diabetes and Exercise Study (IDES)_2 trial belonging to the first and fourth quartile of SED-time were compared. Urine samples were analyzed by comprehensive two-dimensional gas chromatography(GC×GC) with parallel detection by mass spectrometry and flame ionization detection(GC×2GC-MS/FID). This platform enables accurate profiling and fingerprinting of urinary metabolites while maximizing the overall information capacity, quantitation reliability, and response linearity. Moreover, using advanced pattern recognition, the fingerprinting process was extended to untargeted and targeted features, revealing diagnostic urinary fingerprints between groups. Quantitative metabolomics was then applied to analytes of relevance for robust comparisons. Increased levels of glycine, L-valine,L-threonine, L-phenylalanine, L-leucine, L-alanine, succinic acid, 2-ketoglutaric acid, xylitol, and ribitol were revealed in samples from less sedentary women. In conclusion, SED-time is associated with changes in urine metabolome signatures. These preliminary results suggest that reducing SED-time could be a strategy to improve the health status of a large proportion of diabetic patients.

Sedentariness and urinary metabolite profile in type 2 Diabetic Patients, a cross-sectional study / Benetti, Elisa; Liberto, Erica; Bressanello, Davide; Bordano, Valentina; Rosa, Arianna C; Miglio, Gianluca; Haxhi, Jonida; Pugliese, Giuseppe; Balducci, Stefano; Cordero, Chiara. - In: METABOLITES. - ISSN 2218-1989. - 10:5(2020). [10.3390/metabo10050205]

Sedentariness and urinary metabolite profile in type 2 Diabetic Patients, a cross-sectional study

Haxhi, Jonida;Pugliese, Giuseppe;
2020

Abstract

Recent findings indicate a significant association between sedentary (SED)-time and type 2 diabetes mellitus(T2DM). The aim of this study was to investigate whether different levels of SED-time could impact on biochemical and physiological processes occurring in sedentary and physically inactive T2DM patients. In particular, patients from the "Italian Diabetes and Exercise Study (IDES)_2 trial belonging to the first and fourth quartile of SED-time were compared. Urine samples were analyzed by comprehensive two-dimensional gas chromatography(GC×GC) with parallel detection by mass spectrometry and flame ionization detection(GC×2GC-MS/FID). This platform enables accurate profiling and fingerprinting of urinary metabolites while maximizing the overall information capacity, quantitation reliability, and response linearity. Moreover, using advanced pattern recognition, the fingerprinting process was extended to untargeted and targeted features, revealing diagnostic urinary fingerprints between groups. Quantitative metabolomics was then applied to analytes of relevance for robust comparisons. Increased levels of glycine, L-valine,L-threonine, L-phenylalanine, L-leucine, L-alanine, succinic acid, 2-ketoglutaric acid, xylitol, and ribitol were revealed in samples from less sedentary women. In conclusion, SED-time is associated with changes in urine metabolome signatures. These preliminary results suggest that reducing SED-time could be a strategy to improve the health status of a large proportion of diabetic patients.
2020
chemometrics; comprehensive two-dimensional gas chromatography; dual parallel detection by mass spectrometry and flame ionization detector; observational study; sedentariness; type 2 diabetes; urine metabolome
01 Pubblicazione su rivista::01a Articolo in rivista
Sedentariness and urinary metabolite profile in type 2 Diabetic Patients, a cross-sectional study / Benetti, Elisa; Liberto, Erica; Bressanello, Davide; Bordano, Valentina; Rosa, Arianna C; Miglio, Gianluca; Haxhi, Jonida; Pugliese, Giuseppe; Balducci, Stefano; Cordero, Chiara. - In: METABOLITES. - ISSN 2218-1989. - 10:5(2020). [10.3390/metabo10050205]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1414293
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