Analysis of stability in time of marginal adaptation of endosequence root repair material on biological samples

Introduction: The introduction of mineral trioxide aggregate (MTA) and bioceramic sealers increased the success rate of endodontic surgery and perforation repair. The aim of this study was to evaluate the marginal adaptation at different times of endosequence root repair material (ERMM) in order to evaluate its dimensional stability using variable pressure-scanning electron microscope (VP-SEM). Material and Methods: Fourty-eight teeth were selected shaped up to a master apical size of 25. Then a 3mm cut perpendicular to the long axis and a retrograde cavity preparation were performed. In order to obtain 2mm thick sample a second cut was done and, in this disk, ERMM was inserted. The samples were stored at 37°.The samples were divided into four time-depending groups observed with VP-SE Mat time0 (Group 1) andafter2 (Group2),7(Group 3) and 30 days (Group4) after ERRM setting. Statistical analysis with one way-ANOVA test was performed (95%). Results: None of the four groups analyzed showed a complete marginal adaptation between dentin and ERRM. Instead, in all groups ERRM exhibited a completely preserved marginal adaptation to the dentin wall in all time-dependent groups. The mean (±SD) gap value was fortime0,3.91(±2.55) mmafter2days,4.32(±2.69), after7days4.49(±2.53), and after30days4.81(±2.85) mm. No statistically significant difference was found between the four groups. Conclusions: The results of the present study demonstrate the dimensional stability over time of ERMM.