Ferritin proteins are taking center stage as smart nanocarriers for drug delivery due to their hollow cage-like structures and their unique 24-meric assembly. Among all ferritins, the chimeric Archaeoglobus ferritin (HumFt) is able assemble/disassemble varying the ionic strength of the medium while recognizing human TfR1 receptor overexpressed in cancer cells. In this paper we present a highly efficient, large scale purification protocol mainly based on crossflow ultrafiltration, starting from fermented bacterial paste. This procedure allows one to obtain about 2 g of purified protein starting from 100 g of fermented bacterial paste. The current procedure can easily remove contaminant proteins as well as DNA molecules in the absence of expensive and time consuming chromatographic steps.

Application of crossflow ultrafiltration for scaling up the purification of a recombinant ferritin / Palombarini, F.; Ghirga, F.; Boffi, A.; Macone, A.; Bonamore, A.. - In: PROTEIN EXPRESSION AND PURIFICATION. - ISSN 1046-5928. - 163:(2019). [10.1016/j.pep.2019.105451]

Application of crossflow ultrafiltration for scaling up the purification of a recombinant ferritin

Palombarini F.
Primo
Investigation
;
Boffi A.
Funding Acquisition
;
Macone A.
Penultimo
Writing – Original Draft Preparation
;
Bonamore A.
Ultimo
Writing – Original Draft Preparation
2019

Abstract

Ferritin proteins are taking center stage as smart nanocarriers for drug delivery due to their hollow cage-like structures and their unique 24-meric assembly. Among all ferritins, the chimeric Archaeoglobus ferritin (HumFt) is able assemble/disassemble varying the ionic strength of the medium while recognizing human TfR1 receptor overexpressed in cancer cells. In this paper we present a highly efficient, large scale purification protocol mainly based on crossflow ultrafiltration, starting from fermented bacterial paste. This procedure allows one to obtain about 2 g of purified protein starting from 100 g of fermented bacterial paste. The current procedure can easily remove contaminant proteins as well as DNA molecules in the absence of expensive and time consuming chromatographic steps.
2019
crossflow ultrafiltration; diatomaceous earth; ferritins; humanized ferritin; large scale purification
01 Pubblicazione su rivista::01a Articolo in rivista
Application of crossflow ultrafiltration for scaling up the purification of a recombinant ferritin / Palombarini, F.; Ghirga, F.; Boffi, A.; Macone, A.; Bonamore, A.. - In: PROTEIN EXPRESSION AND PURIFICATION. - ISSN 1046-5928. - 163:(2019). [10.1016/j.pep.2019.105451]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1316082
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