Candida species are opportunistic pathogens responsible for a variety of diseases, ranging from skin and mucosal lesions to severe systemic, life-threatening infections. Candida albicans accounts for more than 70% of all Candida infections, however, the clinical relevance of other species such as Candida parapsilosis and Candida krusei are being increasingly recognized. Biofilm-producing yeasts cells acquire an increased resistance to antifungal agents, often leading to therapeutic failure and chronic infection. Conventional methods such as crystal violet (CV) and tetrazolium (XTT) reduction assay, developed to evaluate biofilm formation in Candida species are usually time-consuming, present a high intra- and inter-assay variability of the results and are therefore hardly applicable to routine diagnostics. This study describes an in-vitro assay developed for the measurement of biofilm formation in Candida species based on the clinical Biofilm Ring Test ®(cBRT). We found a significant concordance between the cBRT and both CV (k = 0.74) and XTT (k = 0.62), respectively. Nevertheless, the cBRT resulted more reliable and reproducible than CV and XTT, requiring a minimal sample manipulation and allowing a high throughput assessment, directly on viable cells. The results indicate that the cBRT may provide a suitable, cost-effective technique for routine biofilm testing in clinical microbiology.

The clinical Biofilm Ring Test: A promising tool for the clinical assessment of biofilm-producing Candida species / Di Domenico, Enea Gino; Cavallo, Ilaria; Guembe, Maria; Prignano, Grazia; Gallo, Maria Teresa; Bordignon, Valentina; D'Agosto, Giovanna; Sperduti, Isabella; Toma, Luigi; Ensoli, Fabrizio. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 18:3(2018), pp. 1-8. [10.1093/femsyr/foy025]

The clinical Biofilm Ring Test: A promising tool for the clinical assessment of biofilm-producing Candida species

Di Domenico, Enea Gino
Primo
Project Administration
;
Cavallo, Ilaria
Methodology
;
Sperduti, Isabella
Data Curation
;
Toma, Luigi
Supervision
;
Ensoli, Fabrizio
Ultimo
Validation
2018

Abstract

Candida species are opportunistic pathogens responsible for a variety of diseases, ranging from skin and mucosal lesions to severe systemic, life-threatening infections. Candida albicans accounts for more than 70% of all Candida infections, however, the clinical relevance of other species such as Candida parapsilosis and Candida krusei are being increasingly recognized. Biofilm-producing yeasts cells acquire an increased resistance to antifungal agents, often leading to therapeutic failure and chronic infection. Conventional methods such as crystal violet (CV) and tetrazolium (XTT) reduction assay, developed to evaluate biofilm formation in Candida species are usually time-consuming, present a high intra- and inter-assay variability of the results and are therefore hardly applicable to routine diagnostics. This study describes an in-vitro assay developed for the measurement of biofilm formation in Candida species based on the clinical Biofilm Ring Test ®(cBRT). We found a significant concordance between the cBRT and both CV (k = 0.74) and XTT (k = 0.62), respectively. Nevertheless, the cBRT resulted more reliable and reproducible than CV and XTT, requiring a minimal sample manipulation and allowing a high throughput assessment, directly on viable cells. The results indicate that the cBRT may provide a suitable, cost-effective technique for routine biofilm testing in clinical microbiology.
2018
Biofilm; Candida; Candida albicans; Candida infection; Candidiasis; CBRT; Microbiology; Applied Microbiology and Biotechnology
01 Pubblicazione su rivista::01a Articolo in rivista
The clinical Biofilm Ring Test: A promising tool for the clinical assessment of biofilm-producing Candida species / Di Domenico, Enea Gino; Cavallo, Ilaria; Guembe, Maria; Prignano, Grazia; Gallo, Maria Teresa; Bordignon, Valentina; D'Agosto, Giovanna; Sperduti, Isabella; Toma, Luigi; Ensoli, Fabrizio. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 18:3(2018), pp. 1-8. [10.1093/femsyr/foy025]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1225889
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