Analysis of carotenoids is very complex and demanding in terms of both separation and detection. In this article, an analytical strategy relying on high-performance liquid chromatography-photodiode array detection-tandem mass spectrometry (HPLC-PAD-MS/MS) is presented for the large-scale screening of these phytochemicals. Separation was realized by means of Non-Aqueous Reversed Phase (NARP) chromatography on a triacontyl stationary phase kept at subambient temperature, using a mobile phase compatible with atmospheric pressure chemical ionization (APCI). The standards of 14 analytes were used to optimize the method and to predict the chromatographic behaviour of untargeted carotenoids. MS and MS/MS data, obtained during Information Dependent Acquisition (IDA) experiments, were utilized to set up a sensitive HPLC- selected reaction monitoring (SRM) method. Relative abundance between SRM ion currents (ion ratio) allowed the MS distinction of structural isomers (for example, bicyclic, monocyclic and acyclic isomers), while the identification of geometrical isomers was based on Qratio and fine structure, as calculated from UV–vis spectra. The comparison of LC-PAD- SRM chromatograms, acquired after applying two different extraction procedures (matrix solid-phase dispersion and overnight cold saponification), allowed verifying that sweet peppers are a good source of xanthophylls, prevalently occurring as esterified forms. The overall strategy could identify more than 40 carotenoids in some sweet pepper varieties (Jolly, horn and sweet chili pepper) available on the Italian and European food market.

Large-scale profiling of carotenoids by using non aqueous reversed phase liquid chromatography-photodiode array detection–triple quadrupole linear ion trap mass spectrometry: application to some varieties of sweet pepper (Capsicum annuum L.) / Gentili, Alessandra; DAL BOSCO, Chiara; Fanali, Salvatore; Fanali, Chiara. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 164:(2019), pp. 759-767. [10.1016/j.jpba.2018.11.042]

Large-scale profiling of carotenoids by using non aqueous reversed phase liquid chromatography-photodiode array detection–triple quadrupole linear ion trap mass spectrometry: application to some varieties of sweet pepper (Capsicum annuum L.)

Alessandra Gentili
Primo
Investigation
;
Chiara Dal Bosco
Secondo
Validation
;
2019

Abstract

Analysis of carotenoids is very complex and demanding in terms of both separation and detection. In this article, an analytical strategy relying on high-performance liquid chromatography-photodiode array detection-tandem mass spectrometry (HPLC-PAD-MS/MS) is presented for the large-scale screening of these phytochemicals. Separation was realized by means of Non-Aqueous Reversed Phase (NARP) chromatography on a triacontyl stationary phase kept at subambient temperature, using a mobile phase compatible with atmospheric pressure chemical ionization (APCI). The standards of 14 analytes were used to optimize the method and to predict the chromatographic behaviour of untargeted carotenoids. MS and MS/MS data, obtained during Information Dependent Acquisition (IDA) experiments, were utilized to set up a sensitive HPLC- selected reaction monitoring (SRM) method. Relative abundance between SRM ion currents (ion ratio) allowed the MS distinction of structural isomers (for example, bicyclic, monocyclic and acyclic isomers), while the identification of geometrical isomers was based on Qratio and fine structure, as calculated from UV–vis spectra. The comparison of LC-PAD- SRM chromatograms, acquired after applying two different extraction procedures (matrix solid-phase dispersion and overnight cold saponification), allowed verifying that sweet peppers are a good source of xanthophylls, prevalently occurring as esterified forms. The overall strategy could identify more than 40 carotenoids in some sweet pepper varieties (Jolly, horn and sweet chili pepper) available on the Italian and European food market.
2019
nutraceuticals; carotenoids; non-aqueous reversed-phase chromatography; HPLC-PAD-MS, large-scale profiling; sweet pepper
01 Pubblicazione su rivista::01a Articolo in rivista
Large-scale profiling of carotenoids by using non aqueous reversed phase liquid chromatography-photodiode array detection–triple quadrupole linear ion trap mass spectrometry: application to some varieties of sweet pepper (Capsicum annuum L.) / Gentili, Alessandra; DAL BOSCO, Chiara; Fanali, Salvatore; Fanali, Chiara. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 164:(2019), pp. 759-767. [10.1016/j.jpba.2018.11.042]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1202990
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