Hepatic stem / progenitor cells (HpSCs) are facultative bipotential stem cells, located in Canals of Hering and surrounded by a specialized niche. We aimed to investigate the modulation of HpSC niche and the modification of adipocytokine expression induced by laparoscopic sleeve gastrectomy (LSG) in adolescents with nonalcoholic fatty liver disease (NAFLD). Twenty obese adolescents who underwent LSG and with biopsy-proven NAFLD were included. At baseline (T0) and 1 year after treatment (T1), patients underwent clinical evaluation, blood tests, and liver biopsy. HpSCs, hepatic stellate cells (HSCs), macrophages, and adipocytokines were evaluated by immunohistochemistry and immunofluorescence. Liver biopsies after LSG demonstrated a significant improvement of NAFLD Activity Score and fibrosis. Immunohistochemistry indicated a significant reduction of hepatocyte cell cycle arrest, HpSC activation, activated HSC, and macrophage number after LSG compared with T0. The number of S100A9+ macrophages was also reduced by LSG correlating with resistin expression in HpSC. Finally, serum levels of proinflammatory cytokines significantly correlated with macrophages and activated HSC numbers. The histologic improvement induced by LSG is associated with the reduced activation of local cellular cross-talks, thus, strengthening the role of stem cell niche and hepatic adipocytokine production in the pathogenesis of NAFLD.

Hepatic stem cells and adipocytokines in nonalcoholic fatty liver disease pediatric patients after laparoscopic sleeve gastrectomy / Carpino, Guido; Franchitto, Antonio; Overi, Diletta; Nobili, Valerio; Gaudio, Eugenio. - In: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY. - ISSN 1122-6714. - 123:1 (Supplement)(2018), pp. 45-45. (Intervento presentato al convegno 72° Congresso della Società Italiana di Anatomia e Istologia tenutosi a Parma; Italy).

Hepatic stem cells and adipocytokines in nonalcoholic fatty liver disease pediatric patients after laparoscopic sleeve gastrectomy

Guido Carpino;Antonio Franchitto;Diletta Overi;Valerio Nobili;Eugenio Gaudio
2018

Abstract

Hepatic stem / progenitor cells (HpSCs) are facultative bipotential stem cells, located in Canals of Hering and surrounded by a specialized niche. We aimed to investigate the modulation of HpSC niche and the modification of adipocytokine expression induced by laparoscopic sleeve gastrectomy (LSG) in adolescents with nonalcoholic fatty liver disease (NAFLD). Twenty obese adolescents who underwent LSG and with biopsy-proven NAFLD were included. At baseline (T0) and 1 year after treatment (T1), patients underwent clinical evaluation, blood tests, and liver biopsy. HpSCs, hepatic stellate cells (HSCs), macrophages, and adipocytokines were evaluated by immunohistochemistry and immunofluorescence. Liver biopsies after LSG demonstrated a significant improvement of NAFLD Activity Score and fibrosis. Immunohistochemistry indicated a significant reduction of hepatocyte cell cycle arrest, HpSC activation, activated HSC, and macrophage number after LSG compared with T0. The number of S100A9+ macrophages was also reduced by LSG correlating with resistin expression in HpSC. Finally, serum levels of proinflammatory cytokines significantly correlated with macrophages and activated HSC numbers. The histologic improvement induced by LSG is associated with the reduced activation of local cellular cross-talks, thus, strengthening the role of stem cell niche and hepatic adipocytokine production in the pathogenesis of NAFLD.
2018
72° Congresso della Società Italiana di Anatomia e Istologia
04 Pubblicazione in atti di convegno::04d Abstract in atti di convegno
Hepatic stem cells and adipocytokines in nonalcoholic fatty liver disease pediatric patients after laparoscopic sleeve gastrectomy / Carpino, Guido; Franchitto, Antonio; Overi, Diletta; Nobili, Valerio; Gaudio, Eugenio. - In: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY. - ISSN 1122-6714. - 123:1 (Supplement)(2018), pp. 45-45. (Intervento presentato al convegno 72° Congresso della Società Italiana di Anatomia e Istologia tenutosi a Parma; Italy).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1184418
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