Polycomb repressive complex 2 (PRC2) represents a transcriptional epigenetic repression system, and is responsible of trimethylating histone H3 at lysine 27 (H3K27me3) through its catalytic subunit (EZH2). In plants PRC2 controls diverse developmental processes, from fertilization to flowering. In Arabidopsis, it has been widely described the involvement of PRC2 during endosperm formation, and it was shown that lack of PRC2 lead to embryonic lethality and seed abortion. This has severely hampered studies on the function of PRC2 during seed development. Since in animals EZH2 overexpression and the consequent high level of H3K27me3 are hallmarks of several cancers, several PRC2 specific inhibitors have been designed. Curiously, a pharmacological approach has never been tested on plants, although it may provide a useful tool for the study of PRC2 in plants. Taking advantage of the homology of the catalytic subunit between animals and plants, we have assessed the efficacy of the UNC1999 inhibitor, previously reported to be highly effective in vitro, and of a new selective EZH2 inhibitor on Arabidopsis seeds. Our results showed a reduction of the H3K27me3 mark and a consistent increase in the expression levels of PRC2 targets, thus giving new opportunities to deepen the studies on PRC2.

Selective inhibition of the PRC2 methyltransferase activity suppresses trimethylation of H3K27 in Arabidopsis seeds / Ruta, Veronica; Boccaccini, Alessandra; Longo, Chiara; Madia, VALENTINA NOEMI; Tudino, Valeria; Costi, Roberta; Costantino, Paolo; Vittorioso, Paola. - (2018). (Intervento presentato al convegno International Plant and Molecular Biology 2018- IPMB2018 tenutosi a Montpellier).

Selective inhibition of the PRC2 methyltransferase activity suppresses trimethylation of H3K27 in Arabidopsis seeds

VERONICA RUTA;ALESSANDRA BOCCACCINI;LONGO, CHIARA;VALENTINA NOEMI MADIA;TUDINO, VALERIA;ROBERTA COSTI;PAOLO COSTANTINO;PAOLA VITTORIOSO
2018

Abstract

Polycomb repressive complex 2 (PRC2) represents a transcriptional epigenetic repression system, and is responsible of trimethylating histone H3 at lysine 27 (H3K27me3) through its catalytic subunit (EZH2). In plants PRC2 controls diverse developmental processes, from fertilization to flowering. In Arabidopsis, it has been widely described the involvement of PRC2 during endosperm formation, and it was shown that lack of PRC2 lead to embryonic lethality and seed abortion. This has severely hampered studies on the function of PRC2 during seed development. Since in animals EZH2 overexpression and the consequent high level of H3K27me3 are hallmarks of several cancers, several PRC2 specific inhibitors have been designed. Curiously, a pharmacological approach has never been tested on plants, although it may provide a useful tool for the study of PRC2 in plants. Taking advantage of the homology of the catalytic subunit between animals and plants, we have assessed the efficacy of the UNC1999 inhibitor, previously reported to be highly effective in vitro, and of a new selective EZH2 inhibitor on Arabidopsis seeds. Our results showed a reduction of the H3K27me3 mark and a consistent increase in the expression levels of PRC2 targets, thus giving new opportunities to deepen the studies on PRC2.
2018
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1174521
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