Oligogalacturonides (OGs) are pectic fragments derived from the partial degradation of homogalacturonan in the plant cell wall and able to elicit plant defence responses. Recent methodological advances in the isolation of OGs from plant tissues and their characterization have confirmed their role as bona fide plant Damage-Associated Molecular Patterns. Here, we describe the methods for the isolation of OGs from Arabidopsis leaf tissues and for the characterization of OG structure and biological activity.

Methods of isolation and characterization of oligogalacturonide elicitors / Benedetti, Manuel; Mattei, Benedetta; Pontiggia, Daniela; Salvi, Gianni; Savatin, Daniel Valentin; Ferrari, Simone. - STAMPA. - 1578(2017), pp. 25-38. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-4939-6859-6_3].

Methods of isolation and characterization of oligogalacturonide elicitors

Benedetti, Manuel;Mattei, Benedetta;Pontiggia, Daniela;Savatin, Daniel Valentin;Ferrari, Simone
2017

Abstract

Oligogalacturonides (OGs) are pectic fragments derived from the partial degradation of homogalacturonan in the plant cell wall and able to elicit plant defence responses. Recent methodological advances in the isolation of OGs from plant tissues and their characterization have confirmed their role as bona fide plant Damage-Associated Molecular Patterns. Here, we describe the methods for the isolation of OGs from Arabidopsis leaf tissues and for the characterization of OG structure and biological activity.
2017
Plant Pattern Recognition Receptors: Methods and Protocols
978-1-4939-6858-9
978-1-4939-6859-6
Damage-associated molecular patterns; Elicitors; Innate immunity; Oligogalacturonides; Pectin; Arabidopsis; Arabidopsis Proteins; Cell Wall; Pectins; Peptides; Plant Immunity; Plant Leaves; Protein Conformation; Molecular Biology; Genetics
02 Pubblicazione su volume::02a Capitolo o Articolo
Methods of isolation and characterization of oligogalacturonide elicitors / Benedetti, Manuel; Mattei, Benedetta; Pontiggia, Daniela; Salvi, Gianni; Savatin, Daniel Valentin; Ferrari, Simone. - STAMPA. - 1578(2017), pp. 25-38. - METHODS IN MOLECULAR BIOLOGY. [10.1007/978-1-4939-6859-6_3].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1085904
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