Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.

Preparation of rat hippocampal organotypic cultures and application to study amyloid beta-peptide toxicity / Bronzuoli, Maria Rosanna; Facchinetti, Roberta; Scuderi, Caterina. - STAMPA. - 1727(2018), pp. 333-341. [10.1007/978-1-4939-7571-6_24].

Preparation of rat hippocampal organotypic cultures and application to study amyloid beta-peptide toxicity

Bronzuoli, Maria Rosanna;Facchinetti, Roberta;Scuderi, Caterina
2018

Abstract

Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.
2018
Methods in Molecular Biology
978-1-4939-7570-9
brain slices; ex vivo; hippocampus; neuropharmacology; organotypic culture; rat; toxicity; beta-amyloid; molecular biology; genetics
02 Pubblicazione su volume::02a Capitolo o Articolo
Preparation of rat hippocampal organotypic cultures and application to study amyloid beta-peptide toxicity / Bronzuoli, Maria Rosanna; Facchinetti, Roberta; Scuderi, Caterina. - STAMPA. - 1727(2018), pp. 333-341. [10.1007/978-1-4939-7571-6_24].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1083614
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