We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices / Rizzo, Riccardo; Alvaro, Maria; Danz, Norbert; Napione, Lucia; Descrovi, Emiliano; Schmieder, Stefan; Sinibaldi, Alberto; Chandrawati, Rona; Rana, Subinoy; Munzert, Peter; Schubert, Thomas; Maillart, Emmanuel; Anopchenko, Aleksei; Rivolo, Paola; Mascioletti, Alessandro; Sonntag, Frank; Stevens, Molly M.; Bussolino, Federico; Michelotti, Francesco. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 255:(2018), pp. 2143-2150. [10.1016/j.snb.2017.09.018]

Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Sinibaldi, Alberto;Anopchenko, Aleksei;Michelotti, Francesco
2018

Abstract

We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.
2018
Biosensor platform; Bloch surface waves; Complex matrices; Enhanced fluorescence; Label-free optical sensor; Tumor biomarker; Electronic, Optical and Magnetic Materials; Instrumentation; Condensed Matter Physics; Surfaces, Coatings and Films; 2506; Electrical and Electronic Engineering; Materials Chemistry2506 Metals and Alloys
01 Pubblicazione su rivista::01a Articolo in rivista
Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices / Rizzo, Riccardo; Alvaro, Maria; Danz, Norbert; Napione, Lucia; Descrovi, Emiliano; Schmieder, Stefan; Sinibaldi, Alberto; Chandrawati, Rona; Rana, Subinoy; Munzert, Peter; Schubert, Thomas; Maillart, Emmanuel; Anopchenko, Aleksei; Rivolo, Paola; Mascioletti, Alessandro; Sonntag, Frank; Stevens, Molly M.; Bussolino, Federico; Michelotti, Francesco. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - STAMPA. - 255:(2018), pp. 2143-2150. [10.1016/j.snb.2017.09.018]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1074171
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