AIM: An attempt was made to improve the standardization of sperm motility assessment. METHODS: A computerized system based on image superimposition producing final image with a motion effect was employed. Numerical definition of straight-line velocity (VSL), curvilinear velocity (VCL) and linearity (LIN) related to these motility classes were made, at different frame/rates (20 frames/s, 30 frames/s and 60 frames/s). RESULTS: At least 4 motility class categories were found to be necessary (instead of 3, WHO) for a valid assessment. According to the cut-offs suggested by the ROC curves, motility classes were defined as follows: Class 1 (straight progressive motility): VSL = or > 23 microm/s; LIN = or > 0.58 at 20 frames/s; LIN = or > 0.50 at 30 frames/s; LIN = or > 0.44 at 60 frames/s. Class 2 (straight slow motility): VSL > 10 microm/s and < 23 microm/s; LIN = or > 0.58 at 20 frames/s; LIN = or > 0.51 at 30 frames/s; LIN = or > 0.43 at 60 frames/s. Class 3 (progressive non straight motility): VSL > 10 microm/s; LIN < 0.58 at 20 frames/s; LIN < 0.51 at 30 frames/s; LIN<0.43 at 60 frames/s. Class 4 (non progressive motility): VSL <10 microm/s. A frame rate of 20 frames/s was found to be sufficient to distinguish sperm motility classes in standard semen analysis. CONCLUSIONS: The numerical definition of sperm motility classes may contribute towards standardization in the objective evaluation of sperm kinematics.

An attempt to improve standardization of sperm motility class assessment using the superimposed image analysis system (SIAS) software / Mazzilli, Fernando; Delfino, M; Imbrogno, N; Elia, J; Balducelli, F; Rossi, T.. - In: MINERVA UROLOGICA E NEFROLOGICA. - ISSN 0393-2249. - (2006).

An attempt to improve standardization of sperm motility class assessment using the superimposed image analysis system (SIAS) software

MAZZILLI, Fernando;
2006

Abstract

AIM: An attempt was made to improve the standardization of sperm motility assessment. METHODS: A computerized system based on image superimposition producing final image with a motion effect was employed. Numerical definition of straight-line velocity (VSL), curvilinear velocity (VCL) and linearity (LIN) related to these motility classes were made, at different frame/rates (20 frames/s, 30 frames/s and 60 frames/s). RESULTS: At least 4 motility class categories were found to be necessary (instead of 3, WHO) for a valid assessment. According to the cut-offs suggested by the ROC curves, motility classes were defined as follows: Class 1 (straight progressive motility): VSL = or > 23 microm/s; LIN = or > 0.58 at 20 frames/s; LIN = or > 0.50 at 30 frames/s; LIN = or > 0.44 at 60 frames/s. Class 2 (straight slow motility): VSL > 10 microm/s and < 23 microm/s; LIN = or > 0.58 at 20 frames/s; LIN = or > 0.51 at 30 frames/s; LIN = or > 0.43 at 60 frames/s. Class 3 (progressive non straight motility): VSL > 10 microm/s; LIN < 0.58 at 20 frames/s; LIN < 0.51 at 30 frames/s; LIN<0.43 at 60 frames/s. Class 4 (non progressive motility): VSL <10 microm/s. A frame rate of 20 frames/s was found to be sufficient to distinguish sperm motility classes in standard semen analysis. CONCLUSIONS: The numerical definition of sperm motility classes may contribute towards standardization in the objective evaluation of sperm kinematics.
2006
01 Pubblicazione su rivista::01a Articolo in rivista
An attempt to improve standardization of sperm motility class assessment using the superimposed image analysis system (SIAS) software / Mazzilli, Fernando; Delfino, M; Imbrogno, N; Elia, J; Balducelli, F; Rossi, T.. - In: MINERVA UROLOGICA E NEFROLOGICA. - ISSN 0393-2249. - (2006).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/106404
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